Ballistic transformation and Supplement

Dissection, staining, in situ

Gonad dissections

Gonad fixation

DAPI staining

Antibody staining of dissected gonads

Antibody staining for activated MPK-1 (MAPK-YT, Diphospho-MPK-1)

Germline in situ hybridization

Worm powder

RNAi stuff

RNAi plates with lactose (instead of IPTG)

Feeding RNAi

dsRNA (double-stranded RNA) for injection

Genomic stuff

Worm genomic DNA prep

Single worm PCR

Mutagenesis stuff

Mutagenesis with EMS

Mutagenesis with ENU

Transformation stuff

Yeast transformation

Electrocompetent cells

Heat shock transformation (DH10B homemade)

Protein stuff

BSA conjugation with maleimide (Pierce)

Glycine elution (affinity purification)

IPTG induction

Kinase assay

Cytosolic extract & immunoprecipitation

Cytosolic extract using sonication (fast)