Supplemental Page for

Identification of in vivo mRNA targets of GLD-1, a maxi-KH motif containing protein required for C. elegans germ cell development

Min-Ho Lee and Tim Schedl
 
 
 
 
 
 

1. Additional Methods and Materials

2. GLD-1 mediated translational repression of RME-2 results in yolk uptake only in large oocytes (Photo)

3. Yolk accumulates prematurely in gld-1 (null) (Photo)

4. UV-crosslink with Probe 9 (5'-end) and Probe 11 (3'-end) of rme-2 mRNA (Photo)

5.RME-2 expression is regulated normally in fog-2 (null) (Photo)

6. GLD-1 mediated repression of RME-2 expression is conserved in C. briggsae and C. remanei (Photo)

7. GLD-1/FLAG is expressed at the appropriate time and place to rescue gld-1 null (Photo)

8. rme-2 mRNA is expressed at L4 larval stage but RME-2 protein is not expressed (photo)

9. RME-2 expression is regulated normally in gld-1 (q126/q485) female (Photo)

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