<%@LANGUAGE="JAVASCRIPT" CODEPAGE="65001"%> Mark Johnston Lab - Washington University Department of Genetics
News Staff Projects Publications Links ContactUs

CURRICULUM VITAE
Henry Mark Johnston

Date: December 14, 2006


Date of Birth: December 20, 1951


Place of Birth: Stevens Point, Wisconsin

Citizenship: American

Office Address: Department of Genetics
Washington University School of Medicine
4444 Forest Park Blvd., Campus Box 8510
St. Louis, MO 63108
Phone (314) 362-2735Fax (314) 362-2157
Internet: mj@genetics. wustl. edu		 Home Address: 6928 Amherst
St. Louis, MO 63130
(314) 726-5997

 

Education:

1974 Bachelor of Arts, University of Wisconsin, Madison. Field of Study: Molecular Biology
Thesis: Genetics of Nitrogenase of K. pneumoniae; Mentor: Winston Brill

1980 Doctor of Philosophy, University of California, Berkeley. Field of Study: Mol. Biology
Thesis: Regulation of the his Operon of S. typhimurium; Mentor: John Roth

1980 - 83 Postdoctoral Fellow, Department of Biochemistry, Stanford University School of Medicine
Research: Molecular genetics of yeast GAL genes; Mentor: Ronald Davis

Academic Positions:

1983 - 89 Assistant Professor of Genetics Department of Genetics, Washington University School of Medicine
1990 - 97 Associate Professor of Genetics Department of Genetics, Washington University School of Medicine
1997 - Professor of Genetics Department of Genetics, Washington University School of Medicine
2002 - 06 Acting Chairman Department of Genetics, Washington University School of Medicine

Honors and Awards:

2006 Fellow, American Association for the Advancement of Science (AAAS)
2004 Club Champion (B flight), Gateway National Golf Links
2002 Mid-career award, Yeast Genetics & Molecular Biology Meeting
1998 Fellow, American Academy of Microbiology
1985 - 90 Established Investigator, American Heart Association
1981 - 83 Postdoctoral Fellowship, National Institutes of Health
1980 - 81 Postdoctoral Fellowship, National Science Foundation
1975 - 78 Regents Intern Fellowship, University of California

Professional Service:

2003 - 05 President, (and Vice President, Past President), Genetics Society of America
2000 - 02 Member, Scientific Advisory Board, Paradigm Genetics, Inc, RTP, NC
1998 - 2002 Member, Advisory Board, Saccharomyces Genome Database
1996 - 98 Member, Scientific Advisory Board, Acacia Biosciences, Richmond, CA
1996 - 98 Co-chairman, Yeast Genetics and Molecular Biology International Meeting
1995 - 99 Member, NIH Microbial Physiology and Genetics I Study Section

Editorial Service:

2006 Senior Editor, Genetics
2003 - 05 Communicating Editor, Molecular Genetics and Genomics
2001 - Editorial Board, Eukaryotic Cell
 2000 - Editorial Board, Yeast
2000 - Editorial Board, Functional and Integrative Genomics
1995 - 2002 Editorial Board, Genome Research
1992 - Associate Editor, Genetics
1989 - 2000 Editorial Board, Molecular & Cellular Biology

Professional Memberships:

Genetics Society of America
American Society for Microbiology
American Association for the Advancement of Science
American Society for Cell Biology

Grants (PI only):

1983 - 2008   National Institutes of Health #2 RO1 GM32540; P. I. Mark Johnston
Title: Glucose Sensing and Signaling in Yeast. Total Direct Costs 2004-2008: $1,000,000

2001 - 2005   National Institutes of Health #1 RO1 GM63803-01; P. I. Mark Johnston
Title: Comparative DNA sequence analysis of the yeast genome. Total Direct Costs 2001-2005: $1,126,639

1997 - 2000   National Institutes of Health #5 R01 HG0162702; P. I. Mark Johnston Title: Generation of the Complete Set of Yeast Gene Disruptions. Total Direct Costs: $1,961,396

Publications

  1. St. John RT, Johnston HM, Seidman C, Garfinkel D, Gordon JK, Shah VK, Brill WJ: Biochemistry and genetics of Klebsiella pneumoniae strains unable to fix N2. J. Bacteriol. 1975; 121: 759-767.

  2. Hoppe I, Johnston HM, Biek D, Roth JR: A refined map of the hisG gene of S. typhimurium. Genetics 1979; 92: 20-32.

  3. Johnston HM and Roth JR: Histidine mutants requiring adenine: Selection of mutants with reduced hsG expression in Salmonella typhimurium. Genetics 1979; 92: 1-19.

  4. Johnston HM and Roth JR: UGA suppressor that maps within a cluster of ribosomal protein genes. J. Bacteriol. 1980; 144: 300-305.

  5. Johnston H, Barnes WM, Chumley FG, Bossi L, Roth J: The Mechanism for regulation of the histidine operon of Salmonella typhimurium. Proc. Natl. Acad. Sci. USA. 1980; 77: 508-512. (Reprinted in The Operon, Miller and Reznikoff, eds. , Cold Spring Harbor Press).

  6. Johnston HM and Roth JR: DNA sequence changes of mutations altering attenuation control of the histidine operon of Salmonella typhimurium. J. Mol. Biol. 1981; 145: 735-756.

  7. Johnston HM and Roth JR: Genetic analysis of the histidine operon control region of Salmonella typhimurium. J. Mol. Biol. 1981; 145: 713-734.

  8. Johnston M and Davis RW: Sequences that regulate the divergent GAL1-GAL10 promoter in bakers' yeast. Mol. Cell. Biol. 1984; 4: 1440-1448.

  9. Yocum RR and Johnston M: Molecular cloning of the GAL80 gene from Saccharomyces cerevisiae and characterization of a gal80 deletion. Gene 1984; 2: 75-82.

  10. Johnston M and Dover J: Mutations that inactivate a yeast transcriptional regulatory protein cluster in an evolutionarily conserved DNA binding domain. Proc. Natl. Acad. Sci. USA. 1987; 84: 2401-2405.

  11. JOHNSTON M: Genetic evidence that zinc is an essential co-factor in the DNA binding domain of GAL4 protein. Nature 1987; 328: 353-355.

  12. Orser CS, Goodner BW, Johnston M, Gelvin SB, Csonka LN: The Escherichia coli proB gene corrects the proline auxotrophy of Saccharomyces cerevisiae pro1 mutants. Mol. Gen. Genet. 1988; 212: 124-128.

  13. Johnston M and Dover J: Mutational analysis of the gal4-encoded transcriptional regulatory protein of Saccharomyces cerevisiae. Genetics 1988; 120: 63-74.

  14. Hovland P, Flick J, JOHNSTON M, Sclafani RA: Galactose as a gratuitous inducer of GAL gene expression in yeasts growing on glucose. Gene 1989; 83: 57-64.

  15. Mylin LM, JOHNSTON M, Hopper J: Phosphorylated forms of GAL4 are correlated with ability to activate transcription. Mol. Cell. Biol. 1990; 10: 4623-4629.

  16. Flick J and Johnston M: Two systems of glucose repression of the GAL1 promoter in S. cerevisiae. Mol. Cell. Biol. 1990; 10: 4757-4769.

  17. Nogae I and Johnston M: Isolation and characterization of the ZWF1 gene encoding glucose-6-phosphate dehydrogenase of Saccharomyces cerevisiae. Gene 1990; 96: 161-169.

  18. Wilson TE, Fahrner TJ, JOHNSTON M, Milbrandt J: Identification of the DNA binding site of NGFI-B using genetic selection in yeast. Science 1991; 252: 1297-1300.

  19. Griggs D and JOHNSTON M: Regulated expression of the GAL4 transcriptional activator provides a sensitive genetic switch for glucose repression in yeast. Proc. Nat. Acac. Sci. USA . 1991; 88: 8597-8601.

  20. Flick J and JOHNSTON M: GRR1 of S. cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats. Mol. Cell. Biol. 1991; 11: 5101-5112.

  21. Flick J and JOHNSTON M: Analysis of URS-mediated glucose repression of the GAL1 promoter of Saccharomyces cerevisiae. Genetics 1992; 130: 295-304.

  22. Wilson TE, Day ML, Pexton T, Padgett KA, JOHNSTON M, Milbrandt J: In Vivo Mutational Analysis of the NGFI-A Zinc Fingers. J. Biol. Chem. 1992; 267: 3718-3724.

  23. Erickson JR and JOHNSTON M: Direct cloning of yeast genes from an ordered set of lambda clones in Saccharomyces cerevisiae by recombination in vivo. . Genetics 1992; 134: 151-157.

  24. Griggs D and JOHNSTON M: Promoter elements determining weak expression of the GAL4 regulatory gene of Saccharomyces cerevisiae. Mol. Cell. Biol. 1993; 13: 4999-5009.

  25. Erickson JR and JOHNSTON M: Genetic and molecular characterization of GAL83: Its interaction and similarities with other genes involved in glucose repression in Saccharomyces cerevisiaee. Genetics 1993; 135: 655-664.

  26. Wilson TE, Padgett KE, JOHNSTON M, Milbrandt J: A genetic method for defining DNA binding domains: Application to the Nuclear Receptor NGFI-B. Proc. Nat. Acad. Sci. USA. 1993; 90: 9186-9190.

  27. Liu J, Wilson TE, Milbrandt J, JOHNSTON M: Identifying DNA -binding sites and analyzing DNA-binding domains using a yeast selection system. Methods 1993; 5: 125-137.

  28. Erickson JR and JOHNSTON M: Suppressors reveal two classes of glucose repression genes in the yeast Saccharomyces cerevisiae. Genetics 1994; 136: 1271-1278.

  29. JOHNSTON M, Flick JS, Pexton T: Multiple mechanisms provide rapid and stringent glucose repression of GAL gene expression in Saccharomyces cerevisiae. Mol. Cell. Biol. 1994; 14: 3834-3841.

  30. JOHNSTON M and 34 others. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.  Science 1994; 265: 2077-2082.

  31. Hull MW, Erickson J,JOHNSTON M, Engelke DR: tRNA Genes as Transcriptional Repressor Elements Mol. Cell. Biol. 1994; 14: 1266-1277.

  32. Özcan S and JOHNSTON M: Three different regulatory mechanisms enable yeast hexose transporter (HXT) genes to be induced by different levels of glucose. Mol. Cell. Biol. 1995; 15: 1564-1572.

  33. Hamer L, JOHNSTON M, Green ED: Isolation of yeast artificial chromosomes free of endogenous yeast chromosomes: construction of alternate hosts with defined karyotypic alterations. Proc. Nat. Acad. Sci. USA. 1995; 92: 11706-11710.

  34. Niedenthal RK, Riles L, JOHNSTON M, HegemannJH: Green Fluorescent Protein as a Marker for Gene Expression and Subcellular Localization in Budding Yeast. Yeast 1996; 12: 773-786.

  35. Lutfiyya, LL and JOHNSTON M: Two Zinc-Finger-Containing Repressors are Responsible for GlucoseRepression of SUC2 Expression. Mol. Cell. Biol. 1996; 16: 4790-4797.

  36. Özcan S, Leong T, JOHNSTON M: Rgt1p of S. cerevisiae, a key regulator of glucose-induced genes, is both an activator and repressor of transcription. Mol. Cell. Biol. 1996; 16: 6419-6426.

  37. Özcan S, Dover J, Rosenwald AG, Woelfl S, JOHNSTON M: Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression. Proc. Nat. Acad. Sci. USA. 1996; 93: 12428-12432.

  38. Özcan S and JOHNSTON M: Two different repressors collaborate to restrict expression of yeast glucose transporter genes HXT2 and HXT4 to low levels of glucose.  Mol. Cell. Biol. 1996; 16: 5536-5545.

  39. Özcan S, Vallier LG, Flick JS, Carlson M, JOHNSTON M: Expression of the SUC2 gene of Saccharomyces cerevisiae is inducible by low levels of glucose. Yeast 1996; 13: 127-137.

  40. JOHNSTON M and 56 others. The nucleotide sequence of Saccharomyces cerevisiae chromosome XII. Nature 1997; 387: 87-90.

  41. Li F, and JOHNSTON M: Grr1 of Saccharomyces cerevisiae is connected to the ubiquitin proteolysis machinery though Skp1: coupling glucose sensing to gene expression and the cell cycle. EMBO J. 1997; 6: 101-110.

  42. Devit M, Waddle J, and JOHNSTON M: Regulated nuclear translocation of the Mig1 glucose repressor. Mol. Biol. of the Cell 1997; 8: 1603-1618.

  43. Ozcan S, Dover J, and JOHNSTON M: Glucose sensing and signaling by two glucose receptors in the yeast Saccharomyces cerevisiae. EMBO J. 1998; 17: 2566-2573.

  44. Lutfiyya L, Iyer V R, DeRisi J, DeVit M, Brown P, and JOHNSTON M: Characterization of Three Related Glucose Repressors and Genes They Regulate in Saccharomyces cerevisiae. Genetics 1998; 150: 1377-1391.

  45. Frolova E, JOHNSTON M, and Majors, J: Binding of the glucose-dependent Mig1p repressor to the GAL1 and GAL4 promoters in vivo: regulation by glucose and chromatin structure. NAR. 1999; 27(5): 1350-1358.

  46. DeVit MJ and JOHNSTON M: The nuclear exportin Msn5 is required for nuclear export of the Mig1 glucose repressor of Saccharomyces cerevisiae. Curr. Biol. 1999; 9: 1231-1241.

  47. Winzeler, EA, 49 others, JOHNSTON M, and Davis, RW: Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 1999; 285: 793-972.

  48. Niedenthal, R, Riles, L, Güldener, U, Klein, S, JOHNSTON, M and Hegemann JH: Systematic analysis of S. cerevisiae Chromosome VIII genes. Yeast 1999; 15: 1775-1796.

  49. Uetz, P, Cagney G, Mansfield TA, Judson RS, Knight JkR, Lockshon D, Narayan V, Srinivasan M, Pochart P, Qureshi-Emili A, Li Y, Godwin B, Conover D, Kalbfleisch T, Vijayadamodar G, Yang M, JOHNSTON M, Fields S, and Rothberg JM: A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature 2000; 403: 623-627.

  50. Ostergaard S, Olsson L, JOHNSTON M, Nielsen J: Increasing galactose consumption by Saccharomyces cerevisiae through metabolic engineering of the GAL gene regulatory network.  Nature Biotech. 2000; 18: 1283-1286.

  51. Cliften PF, Hillier LW, Fulton L, Graves T, Miner T, Gish WR, Waterston RH, JOHNSTON M: Surveying Saccharomyces genomes to identify functional elements by comparative DNA sequence analysis. Genome Res. 2001; 11: 1175-1186.

  52. Miller T, Krogan NJ, Dover J, Erdjument-Bromage H, Tempst P, JOHNSTON M, Greenblatt JF, Shilatifard A: COMPASS: a complex of proteins associated with a trithorax-related SET domain protein. Proc Natl Acad Sci. USA. 2001; 98: 12902-7.

  53. Krogan N, Dover J, Khorrami S, Greenblatt JF, Schneider J, JOHNSTON M, Shilatifard A: COMPASS: a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression. J. Biol. Chem. 2002; 277: 10753-5.

  54. Dover J, Schneider J, Tawiah-Boateng MA, Wood A, Dean K, Johnston M, Shilatifard A: Methylation of Histone H3 by COMPASS Requires Ubiquitination of Histone H2B by Rad6. J. Biol. Chem. 2002; 277: 28368-71.

  55. Giaever G, 72 others, JOHNSTON M: Functional Profiling of the S. cerevisiae Genome. Nature 2002; 418: 387-391.

  56. Chen CN, Porubleva L, Shearer G, Svrakic M, Holden LG, Dover JL, JOHNSTON M, Chitnis PR, Kohl DH: Associating protein activities with their genes: rapid identification of a gene encoding a methylglyoxal reductase in the yeast Saccharomyces cerevisiae. Yeast 2003; 20: 545-54.

  57. Krogan NJ, Dover J, Wood A, Schneider J, Heidt J, Boateng MA, Dean K, Ryan OW, Golshani A, JOHNSTON M, Greenblatt JF, Shilatifard A: The Paf1 Complex Is Required for Histone H3 Methylation by COMPASS and Dot1p. Linking Transcriptional Elongation to Histone Methylation. Mol. Cell 2003; 11: 721-9.

  58. Langkjaer RB, Cliften PF, JOHNSTON M, Piskur J: Yeast genome duplication was followed by asynchronous differentiation of duplicated genes. Nature 2003; 421: 848-52.

  59. Wood A, Krogan NJ, Dover J, Schneider J, Heidt J, Boateng MA, Dean K, Golshani A, Zhang Y, Greenblatt JF, JOHNSTON M, Shilatifard A: Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter. Mol. Cell. 2003; 11: 267-74.

  60. Cliften P, Sudarsanam P, Desikan A, Fulton L, Fulton B, Majors J, Waterston R, Cohen BA, JOHNSTON M: Finding Functional Features in Saccharomyces Genomes by Phylogenetic Footprinting, Science 2003; 301: 71-76. (cited 271 times)

  61. Kim J-H, Polish J and JOHNSTON M: Specificity and Regulation of DNA-binding by the Yeast. Glucose Transporter Gene Repressor Rgt1. Mol. Cell. Biol. 2003; 23: 5208–5216.
  62. Riles L, Shaw RJ, JOHNSTON M, Reines D: Large-scale screening of yeast mutants for sensitivity to the IMP dehydrogenase inhibitor 6-azauracil. Yeast 2004; 21(3): 241-8.
  63. Kaniak A, Xue Z, Macool D, Kim JH, JOHNSTON M: Regulatory network connecting two glucose signal transduction pathways in Saccharomyces cerevisiae. Eukaryotic Cell 2004; 3(1): 221-31.
  64. Moriya H, JOHNSTON M: Glucose sensing and signaling in Saccharomyces cerevisiae through the Rgt2 glucose sensor and casein kinase I. Proc. Natl. Acad. Sci. USA. 2004; Feb 10; 101(6): 1572-7.
  65. Polish J, Kim J-H and JOHNSTON M: How the Rgt1 transcription factor of S. cerevisiae is regulated by glucose. Genetics 2005; 169(2): 583-594.
  66. Kim J-H, Brachet V, Moriya H, and JOHNSTON M: Integration of transcriptional and post-translational regulation in a glucose signal transduction pathway in Saccharomyces cerevisiae. Eukaryotic Cell 2006; 5: 167-73.
  67. Cliften PF, Fulton RS, Wilson RK, and JOHNSTON M: After the duplication: gene loss and adaptation in Saccharomyces genomes. Genetics 2006; 172: 863-872.
  68. Ho S-W, Jona G, Chen CT, JOHNSTON M, Snyder M: Linking DNA-binding proteins to their recognition sequences by using protein microarrays. Proc Natl Acad Sci U S A 2006; 103: 9940-5.
  69. Brown V, Sexton JA, JOHNSTON M:  A glucose sensor in Candida albicans. Eukaryotic Cell 2006; 5: 1726-37.
  70. Kim J-H, JOHNSTON M: Two glucose-sensing pathways converge on Rgt1 to regulate expression of glucose transporter genes in Saccharomyces cerevisiae. J Biol Chem. 2006; 281: 26144-9.

Invited Reviews/Book chapters:

A. Johnston M: A model fungal gene regulatory mechanism: The GAL genes of Saccharomyces cerevisiae. Microbiological Reviews 1987; 51: 458-476.

B. JOHNSTON M and Carlson M: Regulation of carbon and phosphate utilization in The Molecular Biology of the Yeast Saccharomyces(B. Jones, J. Pringle, J. Broach, eds. ) Cold Spring Harbor Press, Cold Spring Harbor, N. Y. 1993; 193-281.

C. JOHNSTON M: Genome Sequencing: The complete code for a eukaryotic cell. Current Biology 1996; 6: 500-503.

D. JOHNSTON M: Towards a complete understanding of how a simple eukaryotic cell works. Trends in Genetics 1996; 12: 242.

E. Goffeau A, Barrell, GB, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F. , Hoheisel JD, Jacq C, JOHNSTON M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG: Life with 6000 Genes. Science 1996; 274: 546-567.

F. JOHNSTON M: Gene chips: Array of hope for understanding gene regulation. Current Biology 1998; 8: R171 R174.

G. JOHNSTON M: Feasting, fasting and fermenting: glucose sensing in yeast and other cells: Trends in Genetics 1998; 15: 29-34.

H. Özcan, S. and JOHNSTON M: Function and regulation of yeast hexose transporters. Microbiol. & Mol. Biol. Rev. 1999; 63: 554-569.

I. JOHNSTON M and Fields, S: Grass-roots genomics. Nature Genetics 2000; 24: 5-6

J. JOHNSTON M: The yeast genome: on the road to the golden Age. Curr. Opin. Genet. Dev. 2000; 10: 617-23.

K. Johnston M, Riles L, Hegemann JH: Gene disruption. Meth. Enzymol. 2002; 350: 290-315.

L. Fields S, Johnston M: A crisis in postgenomic nomenclature. Science 2002; 296(5568): 671-2.

M. Johnston M, Stormo GD: Evolution: Heirlooms in the attic.   Science 2003; Nov 7; 302(5647): 997-9.

N. Johnston M and Hieter P:  Genomics. Chapter 14 in Landmark Papers in Yeast Biology (P. Linder, D. Shore and M. N. Hall, eds. ) Cold Spring Harbor Press, 2003.

O. Hughes TR, Robinson MD, Mitsakakis N, JOHNSTON M. The promise of functional genomics: completing the encyclopedia of a cell. Curr. Opin. Microbiol. 2004; 7: 546-54.

P. JOHNSTON M and Kim J-H: Glucose as a hormone: receptor-mediated glucose sensing in the yeast Saccharomyces cerevisiae. Biochem. Soc. Trans. 2005; 33: 247-52.

Q. Fields S, Johnston M: Whither Model Organism Research?  Science 2005; March 25; 307: 1886-1886.

 

 

 
Home News Staff Projects Publications Links Links ContactUs
This page last edited on 2/16/09 • Contact Webmaster • © Washington University, 2008